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在小鼠的心房和心室肌细胞的分离及钾离子通道的录音
JoVE 신문
생물학
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JoVE 신문 생물학
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
DOI:

11:33 min

March 12, 2013

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Chapters

  • 00:05Title
  • 01:37Animal Anesthesia and Organ Harvesting
  • 03:00Preparation of Heart and Langendorff Perfusion
  • 04:03Dissociation of Cardiomyocytes
  • 06:30Preparation of Cardiomyocytes for Cellular Electrophysiology, Biochemical of IF Studies
  • 08:53Cellular Electrophysiology
  • 10:33Results: Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
  • 11:04Conclusion

Summary

자동 번역

Kv通道功能障碍有关的心律失常。为了研究的分子机制,我们利用一个系统的协议,用于隔离的心房和心室的心肌细胞钾通道附属亚基基因敲除小鼠等心律失常。乳鼠心肌细胞,就可以立即用于细胞电生理研究,生化或免疫荧光法(IF)检测。

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