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Chapters
- 00:05Title
- 01:00Embryo Pre-treatment and Hybridization
- 03:21Applying the Antibody
- 04:32Detection of Probe
- 05:30Alcian Blue / Nuclear Fast Red Stain of Elasmobranch Tissue
- 06:59Shh, Hoxa13 and Mucin-rich Globlet Cell Staining
- 07:51Conclusion
By combining methods for RNA whole mount in situ hybridization and histology, gene expression can be linked with cell fate decisions in the developing embryo. These methods have been adapted to marine elasmobranchs and facilitate the use of these animals as model organisms for biomedical, toxicology and comparative studies.
