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JoVE Journal
Immunology and Infection

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Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
 

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

Article DOI: 10.3791/50167-v 09:49 min March 17th, 2013
March 17th, 2013

챕터

요약

There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.

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Keywords: Peyer's Patches Gut-associated Lymphoid Tissues (GALT) Intestinal Immunosurveillance M Cells Follicle-associated Epithelium (FAE) Dendritic Cells (DCs) Macrophages Lymphocytes Flow Cytometry Cryosectioning Immunostaining
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