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An Orthotopic Bladder Cancer Model for Gene Delivery Studies
 
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An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Article DOI: 10.3791/50181-v 07:48 min December 1st, 2013
December 1st, 2013

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Implantation of cancer cells into the organ of origin can serve as a useful preclinical model to evaluate novel therapies. MB49 bladder carcinoma cells can be grown within the bladder following intravesical instillation. This protocol demonstrates catheterization of the mouse bladder for the purpose of tumor implantation and adenoviral delivery.

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Keywords: Orthotopic Bladder Cancer Model MB49 Murine Bladder Carcinoma Cells Catheterization Glycosaminoglycan Layer Trypsin Adenoviral Construct Luciferase Reporter Gene Gene Delivery Studies
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