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米色/布里特细胞的间质血管细胞的分离和分化

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JoVE 신문 생물학

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

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米色/布里特细胞的间质血管细胞的分离和分化

DOI:

10.3791/50191-v

•

07:22 min

•

March 28, 2013

•

Ulrike Liisberg Aune^2,3, Lauren Ruiz, Shingo Kajimura

¹UCSF Diabetes Center and Department of Cell and Tissue Biology,University of California, San Francisco , ²Department of Biology,University of Copenhagen, Denmark, ³National Institute of Nutrition and Seafood Research, Bergen, Norway

Chapters

00:05Title
01:16Adipose Tissue Isolation
02:13Tissue Cutting and Digestion
03:09Preparing Cell Suspension
03:58Plating Cells
04:46Differentiate Cells
05:47Results: Browning of Primary Adipocytes
07:02Conclusion

Summary

자동 번역

主要从小鼠白色脂肪组织中分离出的白色脂肪前体细胞可以分化成浅米色/ BRITE细胞。这里介绍的是一个可靠的细胞模型系统，研究的分子调控“黑”的白色脂肪。

Tags

Beige Adipocytes Brite Adipocytes Stromal Vascular Fraction Preadipocytes UCP1 PPAR-gamma Obesity Treatment Cell Differentiation Primary Adipocytes

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