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Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
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Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

DOI:
10.3791/50198-v

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11:47 min

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February 08, 2013

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, , , , , , ,
1Department of Oncology,Georgetown University, 2Lombardi Comprehensive Cancer Center,Georgetown University, 3Stem Cell Dynamics,Helmholtz Zentrum München – German Research Center for Environmental Health, 4Department of Medicine,Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science,Dankook University

Chapters

  • 00:05Title
  • 01:48Generation of Primary Mammary Epithelial Cell Cultures
  • 03:39Live-cell Imaging
  • 05:56Assessing Cellular Event from Time-lapse Images
  • 07:50Making Single Cell Fate Maps with Timm’s Tracking Tool (TTT)
  • 09:59Representative Results: Colony Formation in BRCA1 Disrupted Cells
  • 11:12Conclusion

Summary

자동 번역

자동 번역

Time-lapse imaging is used to assess behavior of primary preneoplastic mammary epithelial cells derived from genetically engineered mouse models of breast cancer risk to determine if there are correlations between specific behavioral parameters and distinct genetic lesions.

Tags

Keywords: Time-lapse ImagingPrimary Preneoplastic Mammary Epithelial CellsGenetically Engineered Mouse ModelsBreast CancerCell DivisionApoptosisColony FormationCell MorphologySingle-cell TrackingCell Fate Maps

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