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Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling
 

Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling

Article DOI: 10.3791/50218-v • 16:43 min • March 3rd, 2013
March 3rd, 2013 •
*1, *1, 2, 3, 1, 4, 2, 1
1Department of Biomedical Engineering, University of Virginia, 2Department of Biomedical Engineering, California Polytechnic State University, 3Office of Animal Welfare, University of Virginia, 4Department of Biomedical Engineering & Institute for Computational Medicine, Johns Hopkins University
* These authors contributed equally

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요약
Automatic Translation

We demonstrate a novel arterial ligation model in murine spinotrapezius muscle, including a step-by-step procedure and description of required instrumentation. We describe the surgery and relevant outcome measurements relating to vascular network remodeling and functional vasodilation using intravital and confocal microscopy.

Tags

Murine Spinotrapezius Arteriolar Ligation Microvascular Function Microvascular Remodeling Ischemic Injury Revascularization Intravital Microscopy Confocal Microscopy Functional Vasodilation Arteriolar Diameter Muscle Contraction Tissue Harvesting Fixing Immunostaining Studies
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