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“<em>枸橼酸rodentium</em>鼠标产品型号:研究感染性结肠炎的病原体和宿主的贡献
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
DOI:

11:04 min

February 19, 2013

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Chapters

  • 00:05Title
  • 01:35Preparation of Citrobacter rodentium Inoculum and Oral Gavage of Mice
  • 03:03Measuring Colonic Epithelial Barrier Permeability in C. rodentium-infected Mice
  • 04:18Measurement of Bacterial Load in Tissues of C. rodentium-infected Mice
  • 06:06Histological Assessment and Immunofluorescence Staining of Infected Colon Tissues
  • 08:02Results: Representative Overnight Culture Infection Data from a Frozen Glycerol Stock
  • 10:14Conclusion

Summary

자동 번역

枸橼酸rodentium感染提供了一个有价值的模型来研究肠道细菌感染以及宿主的免疫反应和结肠炎的小鼠。该协议概述了屏障的完整性测量,病原体负荷和病理损害,从而为深入鉴定病原体和宿主小鼠的感染性肠炎的贡献。

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