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Giant liposomfremstilling for billedbehandling og Patch-Clamp Elektrofysiologi
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Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

Giant liposomfremstilling for billedbehandling og Patch-Clamp Elektrofysiologi

DOI:
10.3791/50227-v

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09:03 min

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June 21, 2013

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,
1Department of Physiology and Biophysics,University of Washington

Chapters

  • 00:05Title
  • 01:19Deposition of Lipids from Organic Solvents: Classical Protocol
  • 03:13Electroformation of Giant Liposomes
  • 05:19Deposition of Lipids by Controlled Dehydration of Small Liposomes
  • 07:10Results: Electroformation of Giant Liposomes from Small Liposome Dispersions is Compatible with Reconstituted Ion Channel Function
  • 08:32Conclusion

Summary

자동 번역

자동 번역

Færdigtilberedning funktionelle membranproteiner i kæmpeliposomer af definerede sammensætning er en kraftfuld metode, når det kombineres med patch-clamp elektrofysiologi. Dog kan konventionelle kæmpe liposomproduktion være uforeneligt med protein stabilitet. Vi beskriver protokoller til at lave kæmpeliposomer fra rene lipider eller små liposomer indeholdende ionkanaler.

Tags

Giant LiposomesIon ChannelsElectrophysiologyPatch-clampElectroformationLipid MembranesReconstitutionSmall LiposomesDehydrationVapor EquilibriumSaturated Salt Solutions

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