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Introduction to Solid Supported Membrane Based Electrophysiology
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JoVE 신문 생물학
Introduction to Solid Supported Membrane Based Electrophysiology

Introduction to Solid Supported Membrane Based Electrophysiology

DOI:
10.3791/50230-v

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19:56 min

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May 11, 2013

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, , , , ,
1Department of Biophysical Chemistry,Max Planck Institute of Biophysics, 2Buchmann Institute for Molecular Life Sciences,Goethe University Frankfurt

Chapters

  • 00:01Title
  • 00:06Introduction
  • 01:25Background: Measuring principles
  • 02:51Background: The SSM Setup
  • 05:25Experimental procedure: Mounting the cuvette
  • 08:34Experimental procedure: Checking membrane parameters
  • 09:47Experimental procedure: Adding the protein sample
  • 10:53Experimental procedure: Defining the flow protocol
  • 11:48Experimental procedure: Measuring transporter signals
  • 14:12Data Analysis: Reconstruction of the transporter current
  • 15:07Data Analysis: Representative results
  • 17:26Data Analysis: Artifacts & control measurements
  • 19:00Conclusion

Summary

자동 번역

자동 번역

Here we present an electrophysiological method based on solid supported membranes with focus on its applications for the characterization of electrogenic membrane transporters.

Tags

Solid Supported MembraneElectrophysiologyMembrane ProteinProteoliposomesTransporterCapacitive CouplingTransient CurrentsSolution Exchange

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