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Chapters
- 00:05Title
- 00:56Sample Organization
- 02:09Serum and Quality Control (QC) Sample Preparation Procedures
- 03:25UPLC-MS Data Acquisition
- 04:53Metabolite Identification
- 06:03Results: Identification of a Caffeine Metabolite in Human Serum
- 06:51Conclusion
Non-targeted metabolite profiling by ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS) is a powerful technique to investigate metabolism. This article outlines a typical workflow utilized for non-targeted metabolite profiling of serum including sample organization and preparation, data acquisition, data analysis, quality control, and metabolite identification.
