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Chapters
- 00:05Title
- 01:40RNA Folding
- 03:09Chemical Modification of the RNA
- 04:33Reverse Transcription
- 07:08Preparation of Sequencing Ladder and Capillary Electrophoresis
- 09:55ShapeFinder Software
- 11:15Data Normalization
- 11:51Results: RNA Structure Determination using RNA Structure Software
- 12:53Conclusion
High-throughput selective 2' hydroxyl acylation analyzed by primer extension (SHAPE) utilizes a novel chemical probing technology, reverse transcription, capillary electrophoresis and secondary structure prediction software to determine the structures of RNAs from several hundred to several thousand nucleotides at single nucleotide resolution.
