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1Department of Neurology, McKnight Brain Institute,University of Florida , 2Department of Entomology and Nematology,University of Florida , 3Genetics Institute, Department of Molecular Genetics and Microbiology,University of Florida , 4McKnight Brain Institute, Department of Neuroscience, Genetics Institute, Center for Translational Research on Neurodegenerative Diseases, and Center for Movement Disorders and Neurorestoration,University of Florida
Chapters
- 00:05Title
- 01:24Generating a Replicate and Freezing Flies
- 02:42Collection and Lyophilization of Fly Heads
- 03:53RNA-specific Procedures: Total RNA Extraction and mRNA Purification
- 07:23Metabolite-specific Procedures: Methanol-chloroform Extraction
- 09:54Representative Results
- 12:23Conclusion
We describe here the procedures for the extraction and purification of mRNA and metabolites from Drosophila heads. We are applying these techniques to better understand the cellular perturbations underlying neuronal degeneration. These methodologies can be easily scaled and adapted for other "omic" projects.
