JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:19Microcontact Printing of Fibronectin onto PDMS Substrates
- 02:46Maintenance of Double Transgenic Embryonic Stem Cell Lines
- 04:37In vitro Differentiation of Double Transgenic Embryonic Stem Cell Lines and Isolation and Seeding of Cardiac Progenitors
- 08:14Results: Highly Purified Populations of Embryonic Stem Cell Derived Cardiac Progenitors
- 10:51Conclusion
The generation of aligned myocardial tissue is a key requirement for adapting the recent advances in stem cell biology to clinically useful purposes. Herein we describe a microcontact printing approach for the precise control of cell shape and function. Using highly purified populations of embryonic stem cell derived cardiac progenitors, we then generate anisotropic functional myocardial tissue.
