JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 02:17Passivation of Glass Coverslips with PLL-PEG-biotin
- 04:04Fabrication of Stamps for Microcontact Printing
- 04:38Patterning Streptavidin or Adhesive Cues onto Passivated Glass Coverslips
- 06:13Creating Adhesive Gradients onto Streptavidin-pattern Surfaces
- 07:47Loading of Cells with a Soluble Gradient into the Microfluidics Device for Live Cell Imaging
- 09:08Results: Cell Migration in Response to Adhesive and Soluble Gradients
- 12:16Conclusion
A method for the assembly of adhesive and soluble gradients in a microscopy chamber for live cell migration studies is described. The engineered environment combines antifouling surfaces and adhesive tracks with solution gradients and therefore allows one to determine the relative importance of guidance cues.
