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长期沉默的1S Intersectin的在小鼠肺的特异性siRNA,通过阳离子脂质体重复交货。通过电子显微镜的击倒效果评价
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Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
DOI:

15:55 min

June 21, 2013

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Chapters

  • 00:05Title
  • 02:06Prepare Cationic Liposomes and Liposomes:siRNA-ITSN-1 Complexes
  • 05:30Mouse Retro-orbital Vein Injection (Internal Angle of the Eye Socket)
  • 06:39Mouse Lung Perfusion and Tissue Collection for Biochemical Studies
  • 08:23Mouse Lung Tissue Processing for EM
  • 10:21Results: Down-regulation of Intersectin-1 Expression Affects Morphology of Lung Tissue
  • 15:14Conclusion

Summary

자동 번역

重复眼窝的阳离子liposomes/siRNA/ITSN-1s的复合物在小鼠24天,每72小时注射,有效地传递siRNA双链由75%减少-1S ITSN的mR​​NA和蛋白表达的小鼠肺微血管。这种技术是高度重现性的动物模型中,没有不利影响,并避免了死亡。

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