Journal
/
생물학
/
Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

DOI:
10.3791/50317-v

•

09:32 min

•

May 07, 2013

•

, , , , ,
1Institute for Clinical Neurobiology,University of Wuerzburg, 2Department of Synapses – Circuits – Plasticity,Max Planck Institute of Neurobiology, Martinsried, 3Walter Brendel Centre of Experimental Medicine,Ludwig-Maximilians University of Munich

Chapters

  • 00:05Title
  • 02:07Preparation of Live Cell Imaging
  • 02:50Dye Loading of Cells
  • 04:06ER-calcium Live Cell Imaging with an Inverted Laser Scanning Confocal Microscope
  • 05:57Image Processing and Data Analysis
  • 06:38Representative Endoplasmic Reticulum Calcium Imaging Results
  • 08:18Conclusion

Summary

자동 번역

자동 번역

Targeted-esterase induced dye loading (TED) supports the analysis of intracellular calcium store dynamics by fluorescence imaging. The method bases on targeting of a recombinant Carboxylesterase to the endoplasmic reticulum (ER), where it improves the local unmasking of synthetic low-affinity Ca2+ indicator dyes in the ER lumen.

Tags

Keywords: Calcium DynamicsER CalciumTEDTargeted-esterase Induced Dye LoadingCalcium IndicatorsFluo5NMag-Fluo4Mag-Fura2CarboxylesteraseLentivirusLive Cell ImagingER StructureCalcium ReleaseCalcium Refilling

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/50317?article_type=v

Loading Drosophila Nerve Terminals with Calcium Indicators
check_url/kr/50317?article_type=v

In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
check_url/kr/50317?article_type=v

Calcium Imaging of Cortical Neurons using Fura-2 AM
check_url/kr/50317?article_type=v

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
check_url/kr/50317?article_type=v

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
check_url/kr/50317?article_type=v

Expression and Purification of Virus-like Particles for Vaccination
check_url/kr/50317?article_type=v

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
check_url/kr/50317?article_type=v

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
check_url/kr/50317?article_type=v

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
check_url/kr/50317?article_type=v

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Read Article