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Chapters
- 00:05Title
- 01:22Preparation of Surgical Tubing, Vascular Catheters and Chest Wall Window
- 02:09Tracheostomy
- 02:56Venous Catheterization
- 03:43Intravital Mouse Lung Microscopy Surgery
- 05:54Measurement of the Pulmonary Endothelial Surface Layer Thickness
- 07:06Representative ESL Measurement Results
- 08:31Conclusion
The endothelial glycocalyx/endothelial surface layer is ideally studied using intravital microscopy. Intravital microscopy is technically challenging in a moving organ such as the lung. We demonstrate how simultaneous brightfield and fluorescent microscopy may be used to estimate endothelial surface layer thickness in a freely-moving in vivo mouse lung.
