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In vivo meting van de muis Pulmonary Endothelial oppervlaktelaag
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JoVE 신문 의학
In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer
DOI:

08:55 min

February 22, 2013

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Chapters

  • 00:05Title
  • 01:22Preparation of Surgical Tubing, Vascular Catheters and Chest Wall Window
  • 02:09Tracheostomy
  • 02:56Venous Catheterization
  • 03:43Intravital Mouse Lung Microscopy Surgery
  • 05:54Measurement of the Pulmonary Endothelial Surface Layer Thickness
  • 07:06Representative ESL Measurement Results
  • 08:31Conclusion

Summary

자동 번역

De endotheliale glycocalyx / endotheliale oppervlaktelaag is ideaal bestudeerd met behulp van intravitale microscopie. Intravitale microscopie is technisch uitdagend in een bewegend orgaan zoals de longen. We zien hoe gelijktijdige helderveld en fluorescentiemicroscopie kan worden gebruikt om endotheeloppervlak laagdikte schatten in een vrij bewegende<em> In vivo</em> Muis long.

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