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1Department of Physical Medicine and Rehabilitation,VA Greater Los Angeles Healthcare System, 2Department of Pharmacology and Physiology, Institute for Neuroscience,The George Washington University School of Medicine and Health Sciences, 3Division of Genetics, Department of Medicine,Boston Children’s Hospital, 4Howard Hughes Medical Institute,Boston Children’s Hospital, 5Department of Pathology,Boston Children’s Hospital, Harvard Medical School
Chapters
- 00:05Title
- 01:24Micro-capillary Pipette and Embryo Preparations
- 02:47Ventricular CSF Collection
- 05:01Cortical Explant Dissection
- 06:51Transferring and Culturing of Cortical Explant in CSF
- 08:30CSF Collection Characteristics
- 09:20Conclusion
El líquido ventricular cefalorraquídeo (LCR) baña las células de la corteza cerebral y neuroepitelial progenitoras durante el desarrollo temprano del cerebro del embrión. Aquí se describe el método desarrollado para aislar CSF ventricular de embriones de roedores de diferentes edades con el fin de investigar su función biológica. Además, demostramos nuestra disección cerebral cortical explantes y técnica de cultivo que permita el crecimiento de explantes con volúmenes mínimos de medio de cultivo o LCR.
