/

/

评估神经退行性表型<em>果蝇</em>爬坡试验和全脑多巴胺能神经元免疫组化染色

JoVE 신문
신경과학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 신경과학

Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining

Please note that all translations are automatically generated. Click here for the English version.

评估神经退行性表型<em>果蝇</em>爬坡试验和全脑多巴胺能神经元免疫组化染色

DOI:

10.3791/50339-v

•

09:17 min

•

April 24, 2013

•

Maria Cecilia Barone, Dirk Bohmann

¹Department of Biomolecular Genetics,University of Rochester Medical Center

Chapters

00:05Title
01:10Startle-induced Negative Geotaxis Assay
03:14Negative Geotaxis Assay Data Analysis
04:07Dissecting Adult Drosophila Brains for Whole Mounts
05:14Tyrosine Hydroxylase Immunofluorescence of Whole Mount Brains
08:10Investigating the Stress-protective Nrf2 Pathway in a Drosophila Model of PD
08:56Conclusion

Summary

자동 번역

在这里，我们描述了两个实验，已建立了学习年龄依赖性的多巴胺（DA）神经元的神经退行性疾病<em>果蝇</em>：攀登/的惊跳诱导负趋地性试验，允许DA能神经元变性，这是用来识别和计数DA能神经元全脑坐骑酪氨酸羟化酶免疫功能的影响研究。

Tags

Drosophila Dopaminergic Neurons Neurodegenerative Phenotypes Climbing Assay Whole Brain Immunostaining Parkinson’s Disease Alpha-synuclein Tyrosine Hydroxylase

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

小鼠多巴胺和三维胶原基质检测纹状体外植体的解剖和文化

检测蛋白质棕榈酰化培养海马神经元通过免疫沉淀和酰基-生物素交易所（ABE）

嗅觉受体神经元在全山免疫标记<em>果蝇</em>天线

小鼠多巴胺能神经元的原代培养

嗅觉测定的神经退行性疾病的小鼠模型

原发性脑多巴胺能神经元的分离，培养和长期维持胚胎脑鼠类

攀登缺陷的神经退行性疾病的果蝇模型定量分析

评估主要神经再生<em>爪</em>胚胎使用免疫染色

分析突触调制<em>果蝇</em>曝光时间延长后，光光感受器

全细胞电压钳记录的电生理方法<em>果蝇</em>光感受器

Read Article