Recombineering Homologous Recombination Constructs in Drosophila

Arnaldo Carreira-Rosario; Shane Scoggin; Nevine A. Shalaby; Nathan David Williams; P. Robin Hiesinger; Michael Buszczak

doi:10.3791/50346

Journal

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생물학

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重组工程同源重组构建果蝇</em

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Recombineering Homologous Recombination Constructs in Drosophila

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重组工程同源重组构建果蝇</em

DOI:

10.3791/50346-v

•

14:23 min

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July 13, 2013

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Arnaldo Carreira-Rosario, Shane Scoggin, Nevine A. Shalaby, Nathan David Williams, P. Robin Hiesinger³, Michael Buszczak

¹Department of Molecular Biology,University of Texas Southwestern Medical Center at Dallas, ²Department of Physiology,University of Texas Southwestern Medical Center at Dallas, ³Green Center for Systems Biology,University of Texas Southwestern Medical Center at Dallas

Chapters

00:05Title
01:53Selection of BAC Clone and Region to Target
03:04Insert the Homology Arms into P[acman]-KO 1.0
04:40Recombining the Genomic Region of Interest into P[acman]-KO 1.0
08:59Replacing the Genomic Region with the Targeting Cassette
12:30Anticipated Yields
13:42Conclusion

Summary

자동 번역

同源重组技术大大推进果蝇</em遗传学通过分子精确的突变使创建。最近通过的重组工程允许一个操作大块的DNA，并将其转化为果蝇</em 6。这里介绍的方法，这些技术结合起来，以迅速产生大量的同源重组载体。

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