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高通量,自动化的DNA和RNA的提取从临床样品使用TruTip技术常见的液体处理机器人
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
DOI:

10:47 min

June 11, 2013

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Chapters

  • 00:05Title
  • 02:15The Hamilton STAR liquid-handling Robot
  • 02:51Setting Up a 96-well DNA Extraction from Whole Blood
  • 05:02Sample Lysis and Reagent Distribution
  • 07:31gDNA Extraction
  • 09:12Nucleic Acid Extraction, Speed, Fidelity and Purity
  • 10:18Conclusion

Summary

자동 번역

TruTip是一个简单的核酸提取技术,利用该技术,一种多孔性,单片的连接基质的移液管尖端插入。因此,样品制备格式是兼容的液体处理工具,可用于许多介质中,以高通量临床应用和样品类型。

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