Journal
/
의학
/
Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging
JoVE 신문
의학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 의학
Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

DOI:
10.3791/50364-v

•

10:50 min

•

June 29, 2013

•

, , , , , ,
1The Texas Heart Institute at St. Luke’s Episcopal Hospital, 2Department of Vascular Surgery, The Ninth People’s Hospital, School of Medicine,Shanghai Jiao Tong University

Chapters

  • 00:05Title
  • 02:47Priming the Osmotic Pump
  • 03:58Creating Unilateral Hindlimb Ischemia in the Mouse
  • 06:30Imaging Specimen Preparation
  • 09:40Results: Representative Perfusion of the Vasculature in the Murine Hindlimb Ischemia Model
  • 10:27Conclusion

Summary

자동 번역

자동 번역

We show here the in vivo insertion of an osmotic pump for constant local drug delivery and the creation of hindlimb ischemia in a mouse model. Moreover, the hindlimb vasculature is perfused with Microfil, a silicone radiopaque agent, to prepare for micro-computed tomography (micro-CT) imaging.

Tags

Keywords: Osmotic Drug DeliveryIschemic HindlimbMicrofil PerfusionMicro-computed TomographyPeripheral Arterial DiseasePreclinical ResearchAnimal ModelVascular BiologyAngiogenesisVascular Imaging

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/50364?article_type=v

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging
check_url/kr/50364?article_type=v

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography
check_url/kr/50364?article_type=v

Using Micro-computed Tomography for the Assessment of Tumor Development and Follow-up of Response to Treatment in a Mouse Model of Lung Cancer
check_url/kr/50364?article_type=v

Intraluminal Drug Delivery to the Mouse Arteriovenous Fistula Endothelium
check_url/kr/50364?article_type=v

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli
check_url/kr/50364?article_type=v

Method of Direct Segmental Intra-hepatic Delivery Using a Rat Liver Hilar Clamp Model
check_url/kr/50364?article_type=v

Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients
check_url/kr/50364?article_type=v

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
check_url/kr/50364?article_type=v

Direct Drug Delivery to Kidney via the Renal Artery
check_url/kr/50364?article_type=v

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Read Article