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Culturing Primary Rat Inner Medullary Collecting Duct Cells
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Culturing Primary Rat Inner Medullary Collecting Duct Cells

Culturing Primary Rat Inner Medullary Collecting Duct Cells

DOI:
10.3791/50366-v

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06:11 min

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June 21, 2013

•

, , , , , ,
1Anchored Signalling,Max-Delbrück-Center for Molecular Medicine, 2Leibniz Institute for Molecular Pharmacology (FMP), 3Charité University Medicine Berlin

Chapters

  • 00:05Title
  • 01:24Preparation
  • 02:01Cultivation of Primary Rat Inner Medullary Collecting Duct (IMCD) Cells
  • 05:09Results: IMCD Cell Expression
  • 05:39Conclusion

Summary

자동 번역

자동 번역

Arginine-vasopressin (AVP) controls fine-tuning of body water homeostasis through facilitating water reabsorption by renal principal cells. Here, we present a protocol for the cultivation of primary rat inner medullary collecting duct cells suitable for the elucidation of molecular mechanisms underlying AVP-mediated water reabsorption.

Tags

Primary Rat Inner Medullary Collecting Duct CellsArginine-vasopressinV2 ReceptorAquaporin-2CAMPProtein Kinase AWater ReabsorptionDiabetes InsipidusHeart FailureCell Culture Protocol

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