Journal
/
면역학 및 감염병학
/
加速1型糖尿病感应在小鼠体内的过继转移致糖尿病的CD4 + T细胞
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
Please note that all translations are automatically generated. Click here for the English version.

加速1型糖尿病感应在小鼠体内的过继转移致糖尿病的CD4 + T细胞

DOI:
10.3791/50389-v

•

06:27 min

•

May 06, 2013

•

,
1Department of Microbiology & Immunology,Pennsylvania State University College of Medicine

Chapters

  • 00:05Title
  • 01:11Isolation of T Cells from Spleen and Lymph Nodes of BDC2.5 Mice
  • 02:52Fluorescence-activated Cell Sorting of Diabetogenic CD4+ T Cells from BDC2.5 Mice
  • 04:28Adoptive Transfer of Diabetogenic CD4+ T Cells from BDC2.5 Mice
  • 05:12Monitoring Recipient Mice for Hyperglycemia and T1D
  • 05:36Results: Adoptive T-cell Transfer
  • 06:10Conclusion

Summary

자동 번역

자동 번역

我们提供了一个可重复的方法，诱导1型糖尿病（T1D）在小鼠胰岛抗原特异性的，初级的CD4 + T细胞过继转移在两个星期内。

Tags

Accelerated Type 1 DiabetesAdoptive TransferDiabetogenic CD4+ T CellsNOD MouseAutoimmune DiabetesT Cell Receptor BDC2.5NOD.SCID RecipientT1D PathogenesisTherapeutic Interventions

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

实验转移和CTL过继转移免疫小鼠模型

制备和使用艾滋病毒感染主的CD4 + T细胞作为靶细胞，自然杀伤细胞的细胞毒性检测

评估的HIV-1感染的CD4的先天传感<sup> +</sup>性T细胞浆细胞样树突细胞使用<em>体外</em>共培养体系。

尾部皮肤来研究异基因CD4 + T细胞应答的小鼠移植

诱导小鼠肠道炎症由效应CD4的过继转移<sup> +</sup> CD45RB<sup>高</sup> T细胞免疫缺陷小鼠

CD4的制备<sup> +</sup> T细胞通过显微镜分析GD3和GD2神经节苷脂膜表达的

在从幼稚的CD4 + T细胞的人CD4 + FOXP3 +诱导调节性T细胞（iTregs的）使用含有TGF-β-协议的体外分化

诱导麻痹及视觉系统损伤小鼠 T 细胞特异的视神经脊髓炎抗原水通道蛋白-4

立体定向移植人胶质瘤多形性移植小鼠细胞毒性免疫细胞模型的研究

高盐抗原表达树突状细胞的分离和采用转移

Read Article