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Chapters
- 00:05Title
- 01:53Preparing Pancreatic Acinar Cells for Calcium Imaging
- 04:09Measuring Calcium in Pancreatic Acinar Cells
- 05:08Preparation of Pancreatic Acinar Cells for Cell Injury Assays
- 06:30Measuring Lactate Dehydrogenase Leakage
- 07:33Infecting Pancreatic Acinar Cells with Adenovirus
- 08:01Results
- 09:52Conclusion
We describe a reproducible method of preparing mouse pancreatic acinar cells from a mouse for the purpose of examining acinar cell calcium signals and cellular injury with physiologically and pathologically relevant stimuli. A method for adenoviral infection of these cells is also provided.
