JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 02:05General Set Up and Tissue Preparation
- 05:32Patching Cell Bodies of Retinal Ganglion Cells
- 07:20Recordings of Ganglion Cells Using Dynamic Clamp
- 09:12Antibody Staining Against Lucifer Yellow Filled Retinal Ganglion Cells
- 10:14Results: Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
- 11:27Conclusion
Deze video artikel illustreert de set-up, de procedures te cellichamen patchen en hoe u dynamische clamp opnames implementeren van ganglioncellen in zijn geheel-mount muis retinae. Deze techniek maakt het onderzoek de exacte rol prikkelende en remmende synaptische inputs, en hun relatieve omvang en timing neuronale spiking.
