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Chapters
- 00:05Title
- 02:15Feeder-free Maintenance and Passaging of Human iPS Cell Lines
- 03:54Embryoid Bodies Aggregation and Cardiac Induction
- 06:25Beating Areas Isolation and Culture
- 07:48Single Beating Cells Isolation and Analysis
- 10:06Characterizing Contracting Cardiomyocytes Derived from iPS Cells
- 12:36Conclusion
Pluripotent stem cells, either embryonic or induced pluripotent stem (iPS) cells, constitute a valuable source of human differentiated cells, including cardiomyocytes. Here, we will focus on cardiac induction of iPS cells, showing how to use them to obtain functional human cardiomyocytes through an embryoid bodies-based protocol.
