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Chapters
- 00:05Title
- 01:27Liposome Preparation and Detergent-induced Fusion of Vesicles
- 04:15Removal of the Detergents to Form Proteoliposomes
- 06:28Functional Study of the Ion Channel Activities in Black Lipid Membranes
- 08:49Results: Two-Dimensional Crystallization of KvAP in Membranes
- 09:56Conclusion
Procedures for complete reconstitution of a prototype voltage-gated potassium channel into lipid membranes are described. The reconstituted channels are suitable for biochemical assays, electrical recordings, ligand screening and electron crystallographic studies. These methods may have general applications to the structural and functional studies of other membrane proteins.
