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A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
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A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

DOI:
10.3791/50439-v

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13:22 min

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September 10, 2013

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, , , , ,
1Institute of Toxicology and Genetics,Karlsruhe Institute of Technology – Campus North, 2Institute of Organic Chemistry,Karlsruhe Institute of Technology – Campus North, 3Institute of Organic Chemistry,Karlsruhe Institute of Technology – Campus South

Chapters

  • 00:05Title
  • 02:22Protocol
  • 04:40Drug Treatment of Fish Larvae
  • 07:09Luminescence Recording
  • 07:52Data Analysis with Representative Results
  • 12:01Conclusion

Summary

자동 번역

자동 번역

We describe the procedure and data analysis of a chemical screening system for glucocorticoid stress hormone signaling using zebrafish larvae: the Glucocorticoid Responsive In vivo Zebrafish Luciferase activitY (GRIZLY) assay. The assay sensitively and specifically detects effects on glucocorticoid signaling by compounds that require metabolization or affect endogenous glucocorticoid production.

Tags

Glucocorticoid SignalingZebrafish LarvaLuciferase Reporter SystemChemical ScreeningGRIZLY AssayIn VivoHigh-throughput ScreeningEndocrine DisruptorsStress Research

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