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机械刺激引起的钙波传播的细胞单层：牛角膜内皮细胞的例子

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Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

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机械刺激引起的钙波传播的细胞单层：牛角膜内皮细胞的例子

DOI:

10.3791/50443-v

•

10:46 min

•

July 16, 2013

•

Catheleyne D’hondt, Bernard Himpens, Geert Bultynck

¹Department of Cellular and Molecular Medicine, Laboratory of Molecular and Cellular Signaling,KU Leuven

Chapters

00:05Title
02:38Isolation of Corneal Endothelial Cells
04:56Culture of Bovine Corneal Endothelial Cells
05:56Mechanical Stimulation for Inducing Calcium Wave
07:44Results: Calcium Wave Propagation in Bovine Corneal Endothelial Cells
09:39Conclusion

Summary

자동 번역

细胞间钙<sup> +</sup>波驱动的间隙连接通道和半管。在这里，我们描述了一种方法来测量细胞间钙<sup> +</sup>波在细胞模型在响应到本地单细胞的机械刺激和调查中的应用性能和调节间隙连接通道和半管。

Tags

Intercellular Communication Calcium Wave Propagation Mechanical Stimulation Corneal Endothelial Cells Gap Junction Channels Hemichannels ATP Release Cx43 Fluo4-AM Micromanipulator

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