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Vorbereitung des Mgm101 Rekombinationsprotein von MBP-based Tagging-Strategie
JoVE 신문
생물학
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JoVE 신문 생물학
Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
DOI:

11:40 min

June 25, 2013

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Chapters

  • 00:05Title
  • 02:10Construction of MBP-Mgm101 Fusion Protein
  • 03:16Expression, Induction, Cell Lysis and DNase I Treatment
  • 05:28Purification on Amylose Column
  • 07:07PreScission Protease Cleavage and Cation Exchange Chromatography
  • 08:42Size Exclusion Chromatography
  • 10:27Analysis of Purified Mgm101
  • 11:23Conclusion

Summary

자동 번역

Die Hefe-Mitochondrien nucleoid Protein, Mgm101, ist ein Rad52-Typ Rekombinationsprotein die große oligomere Ringe bildet. Ein Protokoll wird beschrieben, dass lösliches rekombinantes Mgm101 vorzubereiten mit dem Maltose Binding Protein (MBP)-Tagging-Strategie mit Kationenaustausch und Größenausschlusschromatographie gekoppelt.

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