Journal
/
/
La transducción adenoviral de células T CD4 + naïve a estudiar la diferenciación Treg
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
DOI:

15:33 min

August 13, 2013

,

Chapters

  • 00:05Title
  • 02:03Cloning of a Gene of Interest into an Entry Vector
  • 03:16Generation of the Primary Virus Lysate
  • 05:47Virus Amplification
  • 07:34T Cell Infection
  • 09:01T Cell Activation and Polarization
  • 10:01T Cell Fixation Staining for Flow Cytometry
  • 11:51Infection Does Not Interfere with T Cell Activation
  • 14:42Conclusion

Summary

자동 번역

Adenoviral la transferencia génica en células T con la expresión transgénica del receptor de adenovirus Coxsackie ingenuo CD4 permite el análisis molecular de la diferenciación de células T reguladoras<em> In vitro</em>.

Related Videos

Read Article