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获取荧光时间推移电影的芽殖酵母和分析单细胞动力学移植物

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Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS

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获取荧光时间推移电影的芽殖酵母和分析单细胞动力学移植物

DOI:

10.3791/50456-v

•

17:01 min

•

July 18, 2013

•

Christopher J. Zopf, Narendra Maheshri

¹Department of Chemical Engineering,Massachusetts Institute of Technology

Chapters

00:05Title
01:36Obtain Fluorescent Microscopy Movies of Single Yeast Cells Growing in a Microfluidic Chamber
06:33Format and Segment Data for Tracking Using the FormatData GUI in MATLAB
09:00Track Cells and Lineages through Time with ProcessTimeSeries GUI, and Curate ID and Lineage Assignments
12:33Exporting Data for Analysis
13:35Results: Analysis of Single-cell Time Series
16:22Conclusion

Summary

자동 번역

我们提出了一个简单的协议来获得生长的酵母细胞的荧光显微镜的电影，和一个基于GUI的软件程序包，提取单细胞的时间序列数据。该分析包括自动沿袭和集成的跟踪数据的目视检查和手工curation分组表决的时间分配。

Tags

Keywords: Fluorescence Time-lapse Microscopy Single-cell Dynamics Gene Expression Budding Yeast GRAFTS MATLAB Microfluidic Culture Device Cell Segmentation Cell Tracking Lineage Analysis Time Series Analysis

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