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在激光诱导轴突病变张力释放测量评估轴突在Piconewton和毫秒级的分辨率对基材的附着力
JoVE 신문
생체공학
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JoVE 신문 생체공학
Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
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在激光诱导轴突病变张力释放测量评估轴突在Piconewton和毫秒级的分辨率对基材的附着力

DOI:
10.3791/50477-v

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09:31 min

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May 27, 2013

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, ,
1Institute of Biophysics,National Research Council of Italy, 2Dipartimento di Sistemi e Informatica,Università di Firenze, 3Department of Neuroscience and Brain Technologies,Istituto Italiano di Tecnologia

Chapters

  • 00:05Title
  • 01:27Laser Microsphere Manipulation
  • 03:31UV and IR Beam Positioning and Optical Tweezer Calibration
  • 04:56Axotomy and Simultaneous Force Measurement
  • 06:50Results: Measurement of Changes in Cytoskeletal Equilibrium Caused by Laser Induced Lesions
  • 08:50Conclusion

Summary

자동 번역

자동 번역

我们测量了张力释放部分病变进行附着膜的轴突的光学捕获探针上的的同时力显微镜测量用激光剥离器通过在一个轴突。所开发的实验协议评估轴突的培养基材的粘附性。

Tags

Laser-induced Axon LesionTension ReleaseAxonal AdhesionForce SpectroscopyInterferometric TrackingOptical TrappingCell-substrate CouplingNeurite GrowthNeuronal Regeneration

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