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胰蛋白酶精华协议分析视网膜血管小鼠模型

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Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model

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胰蛋白酶精华协议分析视网膜血管小鼠模型

DOI:

10.3791/50489-v

•

09:03 min

•

June 13, 2013

•

Jonathan C. Chou, Stuart D. Rollins, Amani A. Fawzi

¹Department of Ophthalmology,Northwestern University Feinberg School of Medicine

Chapters

00:05Title
01:34Retinal Preparation
02:22Water Washes and Trypsin Digest
03:33Separating the Vasculature
05:32Flat-mounting the Vasculature
06:13Results: Representative Images of Trypsin Digest
08:07Conclusion

Summary

자동 번역

胰蛋白酶的消化是一个最常用的方法来分析视网膜血管。这份手稿中详细介绍的方法，包括关键的改动，优化技术，并删除无血管组织，同时保持整体架构的船只。

Tags

Retinal Vasculature Trypsin Digest Mouse Model Flat-mount Retinal Blood Vessels Retinal Vascular Changes Microaneurysms Capillary Degeneration Endothelial To Pericyte Ratios Mouse Eye Retinal Blood Vessels Retinal Blood Vessel Architecture

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