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该<em>在OVO</em> CAM作为一个肉瘤Xenograftmodel的检测

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The In ovo CAM-assay as a Xenograft Model for Sarcoma

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该<em>在OVO</em> CAM作为一个肉瘤Xenograftmodel的检测

DOI:

10.3791/50522-v

•

12:44 min

•

July 17, 2013

•

Gwen M.L. Sys, Lore Lapeire, Nikita Stevens, Herman Favoreel, Ramses Forsyth, Marc Bracke, Olivier De Wever

¹Department of Orthopaedic Surgery and Traumatology,Ghent University Hospital, ²Department of Radiation Oncology and Experimental Cancer Research,Ghent University, ³Department of Virology, Parasitology, and Immunology,Ghent University, ⁴Pathlicon

Chapters

00:05Title
02:19Preparation of Tumor-derived Single Cell Suspensions
03:59Opening of the Eggs
05:21Inoculation Procedure
06:23Imaging and Harvesting of the CAM
07:20Results: Evaluation of the CAM
11:30Conclusion

Summary

자동 번역

该<em>在OVO</em>绒毛尿囊膜（CAM）接枝源性肉瘤的新鲜肿瘤组织中，其单细胞悬液，并永久和瞬态荧光标记的建立肉瘤细胞株。该模型用于研究移植（活力，Ki67的增殖指数，坏死，浸润）和主机（成纤维细胞浸润，血管长入）的行为。

Tags

Sarcoma Xenograft Model Chick Chorioallantoic Membrane (CAM)In Ovo Cell Lines Saos-2 SW1353 Fluorescent Labeling Graft Behavior Host Response Ki67 Proliferation Index Necrosis Vascular Ingrowth ECM Gel

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