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Chapters
- 00:05Title
- 02:05Cell Transfection
- 03:46Cell Preparation for Flow Cytometry, Western Blot, and Immunofluorescence
- 05:12Flow Cytometry and Immunofluorescence Microscopy
- 06:50Data Analysis
- 07:49Assessing AKAP-Lbc and PDE4D3 Interaction Using BiFC-Flow Cytometry
- 10:17Conclusion
Flowcytometrische analyse van Bimoleculaire Fluorescentie Complementatie biedt een hoge doorvoer kwantitatieve methode om eiwit-eiwit interacties te bestuderen. Deze methodologie kan worden toegepast op mapping eiwitbindingsplaatsen en screenen factoren die eiwit-eiwit interactie te bepalen.
