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Measuring Growth and Gene Expression Dynamics of Tumor-Targeted S. Typhimurium Bacteria
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1Health Sciences and Technology,Massachusetts Institute of Technology, 2Department of Bioengineering,University of California, San Diego , 3Biocircuits Institute,University of California, San Diego , 4Molecular Biology Section, Division of Biological Science,University of California, San Diego , 5Broad Institute of Harvard and MIT, 6Department of Medicine,Brigham and Women’s Hospital, 7Electrical Engineering and Computer Science and David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 8Howard Hughes Medical Institute
Chapters
- 00:01Title
- 00:56Cell preparation and subcutaneous injection
- 01:34Subcutaneous injection of cancer cells
- 02:19Bacterial preparation for injection
- 03:01Tail vein injection of bacteria
- 03:44IVIS imaging of bacteria tumors
- 04:11Organ dissection
- 04:43Quantifying bacterial distribution in tumors
- 05:08Discussion and Representative Results
- 07:38Conclusions
The goal of these experiments is to generate quantitative time-course data on the growth and gene expression dynamics of attenuated S. typhimurium bacterial colonies growing inside tumors. This video covers tumor cell preparation and implantation, bacteria preparation and injection, whole-animal luminescence imaging, tumor excision, and bacterial colony counting.
