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Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
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Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

DOI:
10.3791/50543-v

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09:40 min

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July 10, 2013

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, , , ,
1Division of Marine Biology and Fisheries, Rosenstiel School of Marine and Atmospheric Sciences,University of Miami

Chapters

  • 00:05Title
  • 01:44Day One: Cell Preparation
  • 04:12Day Two: Neuron Isolation
  • 08:24Patch Clamp Recordings of Glutamatergic Currents in the Sensory Neurons of Aplysia
  • 08:49Conclusion

Summary

자동 번역

자동 번역

We describe the dissection of the nervous system of the marine sea hare Aplysia after anesthesia, the isolation of neurons for short term-tissue culture, and recordings of single cell ion currents via the patch clamp technique.

Tags

Aplysia CalifornicaSensory NeuronsPatch ClampGlutamatergic CurrentsMarine Gastropod MolluskNervous System FunctionLearning And MemoryNeuronal Co-cultureDissociated NeuronsIon CurrentsBiophysical CharacterizationPleural GangliaBuccal Ganglia

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