JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Institute for Complex Molecular Systems & Laboratory of Macromolecular and Organic Chemistry,Eindhoven University of Technology & NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 2NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR & IIT@NEST,Center for Nanotechnology Innovation, 3NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 4IIT@NEST,Center for Nanotechnology Innovation
Chapters
- 00:05Title
- 02:30Synthesis of the Sensors
- 04:20In vitro pH Measurements
- 05:36Cell Culture and Electroporation
- 06:38pH Sensing in Living HeLa Cells
- 08:47In vivo Sample Preparation
- 10:30pH Imaging in Mouse Brain
- 12:24Representative Analysis of Dendrimer-based pH Sensor Fluorescence
- 15:45Conclusion
Sensores de fluorescencia son herramientas poderosas en ciencias de la vida. Aquí se describe una metodología para sintetizar y utilizar sensores fluorescentes basadas en dendrímeros para medir el pH en las células vivas e in vivo. El andamio dendríticas mejora las propiedades de los tintes fluorescentes conjugados que conducen a propiedades mejoradas de detección.
