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提高蛋白质结晶的成功率随机Microseed矩阵筛选

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Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening

Please note that all translations are automatically generated. Click here for the English version.

提高蛋白质结晶的成功率随机Microseed矩阵筛选

DOI:

10.3791/50548-v

•

12:24 min

•

August 31, 2013

•

Marisa Till, Alice Robson, Matthew J. Byrne, Asha V. Nair, Stefan A. Kolek, Patrick D. Shaw Stewart, Paul R. Race

¹School of Biochemistry,University of Bristol, ²Douglas Instruments

Chapters

00:05Title
01:58Preparation of the Seed Stock
06:09Establishment of Crystallization Trays
08:45Inspection of Crystallization Trays
09:40Representative rMMS Experiment
11:36Conclusion

Summary

자동 번역

在这里，我们描述了随机microseed矩阵筛选的一般方法。这种技术被示为显著增加的蛋白质结晶筛选实验的成功率，减少需要优化，并提供晶体的数据收集和配体浸泡实验的可靠供应。

Tags

Protein Crystallization Random Microseed Matrix Screening RMMS Vapor Diffusion Sitting Drop Hanging Drop 96-well 24-well Liquid Handling Robotics Protein Phase Diagram Metastable Zone Crystal Quality Crystal Supply Soaking Experiments

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