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Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
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Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

DOI:
10.3791/50589-v

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09:20 min

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September 02, 2013

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1Department of Proteomics and Signal Transduction,Max Planck Institute of Biochemistry

Chapters

  • 00:05Title
  • 01:46Preparation of Tissue Slices for Microdissection and Sample Lysis
  • 03:20Sample Processing
  • 04:51Quantification of the Peptides
  • 05:38Fractionation of the Lys-C and Tryptic Peptides
  • 07:29Results: Peptide Quantification
  • 08:21Conclusion

Summary

자동 번역

자동 번역

Archival formalin fixed and paraffin embedded (FFPE) clinical samples are valuable material for investigation of diseases. Here we demonstrate a sample preparation workflow allowing in-depth proteomic analysis of microdissected FFPE tissue.

Tags

Keywords: ProteomicSample PreparationFASPLysCTrypsinPeptide FractionationQuantitative AnalysisBiomarkersDrug Targets

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