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Chapters
- 00:05Title
- 01:17Installation of the Dual Camera System
- 02:47Camera Alignment
- 04:34Preparation of Cells, Reactive Substrate, and Parallel Plate Flow Chamber
- 05:50Two-color Image Acquisition
- 08:27Representative Imaging Results
- 09:59Conclusion
Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are produced.
