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Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
 

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Article DOI: 10.3791/50605-v 13:28 min September 4th, 2013
September 4th, 2013

챕터

요약

A way to gain new insights into the complexity of the brain inflammatory response is presented. We describe immunofluorescence-based protocols followed by three-dimensional confocal analysis to investigate the pattern of co-expression of microglia/macrophage phenotype markers in a mouse model of focal ischemia.

Tags

Keywords: 3D Confocal Analysis Microglia Macrophage Polarization Brain Injury Ischemia M1 M2 CD11b CD68 Ym1
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