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Chapters
- 00:05Title
- 01:24Preparation of the CometChip
- 02:20Loading Cells into the CometChip
- 03:57Dosing and Repair
- 04:55Alkaline Comet Assay
- 06:23Neutral Comet Assay
- 07:50Fluorescent Imaging
- 08:21Results: Quantification of DNA Damage in Human Lymphoblastoid Cells after Exposure to Oxidative Damage
- 10:15Conclusion
Vi beskriver her en platform, der giver comet assay påvisning af DNA-skader med en hidtil uset overførselshastighed. Enheden mønstre pattedyr celler i en microarray og muliggør parallel behandling af 96 prøver. Den tilgang letter analysen af basisniveau DNA-skader eksponering-induceret DNA-skader og DNA reparation kinetik.
