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Chapters
- 00:05Title
- 01:59Microfluidic Platform Fabrication
- 05:17Microdispenser Setup
- 06:53Isolation and Staining of Mouse Islets
- 08:25Simultaneous Oxygen and Glucose Stimulations
- 09:19Results: Intermittent Hypoxia Preconditioning Improves Islet Responses to Hypoxia
- 10:46Conclusion
Microfluidic oxygen control confers more than just convenience and speed over hypoxic chambers for biological experiments. Especially when implemented via diffusion through a membrane, microfluidic oxygen can provide simultaneous liquid and gas phase modulations at the microscale-level. This technique enables dynamic multi-parametric experiments critical for studying islet pathophysiology.
