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Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice
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Chapters
- 00:00Title
- 00:20Preparation of Mycobacterium leprae Suspension
- 04:24Cold Ziehl-Neelsen Stain
- 06:06Viability Determination
- 07:48Freezing/Thawing M. leprae Suspension
- 09:08Mouse Inoculation
- 09:35Results
Mycobacterium leprae, the causative agent of leprosy, does not grow in vitro. We describe an easy to follow protocol to prepare a bacillary suspension to ensure the maintenance of large quantities of M. leprae for a variety of applications. Protocols for propagation by mouse footpad inoculation, evaluation of viability, freezing and thawing bacillary stock are described in detail.
