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优化的协议<em>麻风分枝杆菌</em>应变管理：冷冻库存保持和维护在无胸腺裸鼠
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice
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优化的协议<em>麻风分枝杆菌</em>应变管理：冷冻库存保持和维护在无胸腺裸鼠

DOI:
10.3791/50620-v

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10:32 min

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March 23, 2014

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, , , , , ,
1Department of Pathology,Instituto Lauro de Souza Lima (ILSL), 2Laboratory Animal House,Instituto Lauro de Souza Lima (ILSL), 3Department of Microbiology,Instituto Lauro de Souza Lima (ILSL), 4Department of Pharmacology,Instituto Lauro de Souza Lima (ILSL)

Chapters

  • 00:00Title
  • 00:20Preparation of Mycobacterium leprae Suspension
  • 04:24Cold Ziehl-Neelsen Stain
  • 06:06Viability Determination
  • 07:48Freezing/Thawing M. leprae Suspension
  • 09:08Mouse Inoculation
  • 09:35Results

Summary

자동 번역

자동 번역

麻风分枝杆菌，麻风病的病原体，不长在体外 。我们描述一个易于遵循的协议准备杆菌悬液，以确保大量M的保养麻风用于各种应用。协议通过鼠标脚垫接种，可行性评估，冻融杆菌股票的传播进行了详细的描述。

Tags

Mycobacterium LepraeLeprosyAthymic Nude MiceBacilli PurificationTrypsinZiehl-Neelsen StainingBacillary CountingFrozen Stock PreservationStrain Maintenance

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